Application

Suggested dilution for most applications: 1:50-1:200 Optimal working dilutions must be determined by end user.

Donkey anti-Goat IgG Antibody, Rhodamine conjugate, Species Adsorbed is an antibody against Donkey Goat IgG for use in IF.

Research CategorySecondary & Control Antibodies

Research Sub CategorySecondary Antibodies Adsorbed for Dual Labeling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Lyophilized. Buffer = 0.02 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.1% sodium azide.

RECONSTITUTION:

Reconstitute with 500 µL of sterile distilled water.

RHODAMINE/PROTEIN:

Approximately 0.5 (A550/A280).

Specificity

Goat IgG

WAVELENGTH:

Absorption peak = 550 nm, Emission peak = 570 nm.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.